| چکیده انگلیسی مقاله |
Extended abstract Introduction: Soybean is a plant that has a moderate tolerance to drought stress. Drought stress is one of the most important abiotic stresses affecting soybean production by about 40%. In addition, drought stress reduces seed vigor as a result of stress conditions. Positive effects of mycorrhizal fungi on the increment of dry matter and plant biomass, especially in low-irrigated conditions and in dry areas have been proven. The reason for the increase in crop yield in mycorrhizal inoculated plants is their water balance in water deficit stress conditions and as a result, absorption of water and mineral elements. Rhizobium bacteria, carbohydrates, and other foods are catched from the phloem vessels and the received energy is used to convert nitrogen to ammonium ion and eventually amino acids. Japonicum rhizobium bacterium is not naturally found in soils of Iran and the bacteria should be added to the soil along with seeds. Materials and Methods: This experiment was carried out to determine the germination characteristics, seed vigor and its related traits in soybean Kosar cultivar grown under drought stress conditions and inoculation with mycorrhiza and bacteria with three replications during 2018. In the field experiment, drought stress included optimal irrigation (irrigation after 70 mm evaporation), moderate stress (irrigation after 110 mm evaporation) and severe drought stress (irrigation after 150 mm evaporation from class A evaporation pan), mycorrhizal fungus in three levels, mycorrhiza-free, and inoculation with glomus mosseae, Glomus intraradices and Rhizobium bacterium in two levels, including no inoculation and inoculation with Rhizobium japonicum. Results: The mean comparison showed that the seeds obtained under normal irrigation, inoculation with mycorrhiza and bacteria had the highest dry weight of radicle, plumule and seedling, percentage and germination rate. The lowest electrical conductivity of the seeds and the mean time of germination were obtained under these condition. Severe and moderate drought stress reduced stress tolerance index, root tolerance index and stem tolerance index, seedling vigor index and seed vigor index compared to optimum irrigation about 42-23, 38-18, 30-18, 50-26 and 41-21) percent, respectively. Inoculation with Glomus mosseae and Glomus intraradices increased the seed vigor index, radicle lentgh, plumule lentgh and seedling length compared to non-inoculation with mycorhizal fungi by 48-42, 27-26, 41-37 and 35-33 percent, respectively. Inoculation with Rhizobium japonicum increased radicle lentgh, plumule lentgh and seedling lentgh compared to non-inoculation with bacterium by 21%, 16% and 18%, respectively. The highest water percentage in seedling tissue was obtained under optimum irrigation conditions, inoculation with mycorrhizal fungi and inoculation with rhizobium bacteria. Conclusion: According to the results of this study, in all three different irrigation conditions to improve germination and increase seed vigor, the use of mycorrhiza fungi is effective especially glomus mosseae and inoculation with Rhizobium bacteria. Highlights: Germination characteristics, germination rate and duration of soybean Kosar cultivar on seeds obtained under different irrigation conditions were investigated. The effect of mycorrhiza and bacteria on seed vigor and related traits were evaluated under different levels of drought stress. The damage done to the seed membranes due to the interaction of irrigation and inoculation with mycorrhiza and bacterium was investigated. |